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Archaea, 2
© 2007 Heron Publishing—Victoria, Canada
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A bifunctional archaeal protein that is a component of 30S ribosomal subunits and interacts with C/D box small RNAs

Andrea Ciammaruconi (1), Stefania Gorini (1) and Paola Londei (1, 2, 3)

1. Dpt. Biotecnologie Cellulari ed Ematologia, Università di Roma “La Sapienza” (Policlinico) Viale Regina Elena 324, 00161 Rome, Italy / 2. Dpt. Biochimica, Biologia e Fisica Medica (DIBIFIM), Università degli Studi di Bari (Policlinico) Piazza Giulio Cesare, 70124 Bari, Italy / 3. Corresponding author ([email protected]) / Received March 7, 2007; accepted May 22, 2007; published online June 29, 2007


We have identified a novel archaeal protein that apparently plays two distinct roles in ribosome metabolism. It is a polypeptide of about 18 kDa (termed Rbp18) that binds free cytosolic C/D box sRNAs in vivo and in vitro and behaves as a structural ribosomal protein, specifically a component of the 30S ribosomal subunit. As Rbp18 is selectively present in Crenarcheota and highly thermophilic Euryarchaeota, we propose that it serves to protect C/D box sRNAs from degradation and perhaps to stabilize thermophilic 30S subunits.

Keywords: C/D box sRNA, ribosomal protein, rRNA methylation, Sulfolobus solfataricus.

 ISSN 1472-3654 (Online) ISSN 1472-3646 (Print) Copyright © 2002–2007 Heron Publishing