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Archaea, 1:247–253
© 2004 Heron Publishing—Victoria, Canada
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Interactions between RNase P protein subunits in Archaea

Thomas A. Hall (1) and James W. Brown (2, 3)

1. Ibis Therapeutics, 2292 Faraday Ave., Carlsbad, CA 92008, USA / 2. Department of Microbiology, North Carolina State University, Raleigh, NC 27695, USA / 3. Corresponding author ([email protected]) / Received October 6, 2003; accepted December 22, 2003; published online February 6, 2004


A yeast two-hybrid system was used to identify protein–protein interactions between the ribonuclease P (RNase P) protein subunits Mth11p, Mth687p, Mth688p and Mth1618p from the archaeon Methanothermobacter thermoautotrophicus. Clear interactions between Mth688p and Mth687p, and between Mth1618p and Mth11p, were confirmed by HIS3 and LacZ reporter expression. Weaker interactions of Mth687p and Mth688p with Mth11p, and Mth11p with itself, are also suggested. These interactions resemble, and confirm, those previously seen among the homologs of these proteins in the more complex yeast RNase P holoenzyme.

Keywords: archaebacteria, MTH11, MTH1618, MTH687, MTH688, ribonuclease P.

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